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Free chlorine and phytic acid synergistically inactivated conidia of Aspergillus spp.

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Abstract

Chlorination has been widely used to disinfect various microbials in the environment, but its fungicidal activity is known to be limited. Here, we demonstrate that a combinatorial treatment with free chlorine and phytic acid exerted high fungicidal activities against selected species of Aspergillus. Treatment with either chlorine (7 mg/l) or phytic acid (∼400 mg/l) without pH adjustment caused marginal inactivation of Aspergillus niger conidia within 5 min. However, the combinatorial treatment with free chlorine and phytic acid inactivated 98% of A. niger conidia within 5min (\(\overline {\rm{C}} {\rm{T}} = 25.7\;{\rm{mg/}}l \cdot \min \)). Overall fungicidal efficiency of combinatorial application was higher (∼256%) than the sum of inactivation levels by individual treatment, suggesting a synergistic effect between free chlorine and phytic acid. Transmission electron microscopy observation showed that free chlorine primarily disrupted nucleo-cytosolic organs, whereas phytic acid preferentially disintegrated the cell wall and plasma membrane. The combination of both agents demolished the conidial structure of A. niger. The effects of these chemicals on the cell membrane were verified with propidium iodide staining, lipid peroxidation, and extracellular ATP secretion. Fungicidal activities of chlorine and phytic acid were further confirmed against A. parasiticus and A. flavus. Our data suggest that the mixture of free chlorine and phytic acid without any additional preparation may efficiently disinfect Aspergillus spp. through the synergistic activities of individual components.

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References

  1. J. Guinea, T. Pelaez, L. Alcala and E. Bouza, Med. Mycol., 44, 349 (2006).

    PubMed  Google Scholar 

  2. B. Mousavi, M. T. Hedayati, N. Hedayati, M. Ilkit and S. Syedmousavi, Curr. Med. Mycol., 2, 36 (2016).

    CAS  PubMed  PubMed Central  Google Scholar 

  3. H. Brahm and M. D. Segal, N. Engl. J. Med., 360, 1870 (2009).

    Google Scholar 

  4. P. Kumar, D. K. Mahato, M. Kamle, T. K. Mohanta and S. G. Kang, Front. Microbiol., 7, 2170 (2016).

    PubMed  Google Scholar 

  5. L. Zhu, B. Zhang, Y. Dai, H. Li and W. Xu, Toxins (Basel), 9, 113 (2017).

    Google Scholar 

  6. X. Ma and K. Bibby, Water Res., 120, 265 (2017).

    CAS  PubMed  Google Scholar 

  7. H. Nourmoradi, M. Nikaeen, C. R. Stensvold and H. Mirhendi, Water Res., 46, 5935 (2012).

    CAS  PubMed  Google Scholar 

  8. M. Sokmen, I. Tatlidil, C. Breen, F. Clegg, C. K. Buruk, T. Sivlim and S. Akkan, J. Hazard. Mater., 187, 199 (2011).

    PubMed  Google Scholar 

  9. K. C. Piacentini, G. D. Savi and V. M. Scussel, Qual. Assur. Saf. Crop., 9, 383 (2017).

    CAS  Google Scholar 

  10. R. Hassan, S. El-Kadi and M. Sand, J. Adv. Biol., 2, 1 (2015).

    CAS  Google Scholar 

  11. S. Fukuzaki, Biocontrol. Sci., 11, 147 (2006).

    CAS  PubMed  Google Scholar 

  12. V. J. Pereira, R. Marques, M. Marques, M. J. Benoliel and M. T. Barreto Crespo, Water Res., 47, 517 (2013).

    CAS  PubMed  Google Scholar 

  13. G. Wen, X. Xu, T. Huang, H. Zhu and J. Ma, Water Res., 125, 132 (2017).

    CAS  PubMed  Google Scholar 

  14. M. Sisti, G. Brandi, M. De Santi, L. Rinaldi and G. F. Schiavano, J. Water Health, 10, 11 (2012).

    CAS  PubMed  Google Scholar 

  15. D. Kanzler, W. Buzina, A. Paulitsch, D. Haas, S. Platzer, E. Marth and F. Mascher, Mycoses, 51, 165 (2008).

    CAS  PubMed  Google Scholar 

  16. H. M. Al-Gabr, T. Zheng and X. Yu, Sci. Total. Environ., 463–464, 525 (2013).

    PubMed  Google Scholar 

  17. M. N. Groot, T. Abee and H. van Bokhorst-van de Veen, Food Microbiol., 81, 108 (2019).

    Google Scholar 

  18. J. S. Piotrowski, H. Okada, F. Lu, S. C. Li, L. Hinchman, A. Ranjan, D. L. Smith, A. J. Higbee, A. Ulbrich, J. J. Coon, R. Deshpande, Y. V. Bukhman, S. McIlwain, I. M. Ong, C. L. Myers, C. Boone, R. Landick, J. Ralph, M. Kabbage and Y. Ohya, Proc. Natl. Acad. Sci. USA, 112, E1490 (2015).

    CAS  PubMed  Google Scholar 

  19. C. H. Pohl, J. L. F. Kock and V. S. Thibane, Antifungal free fatty acids: A review, Formatex, Badajoz (2011).

    Google Scholar 

  20. J. Yun and D. G. Lee, FEMS Yeast Res., 16, 1 (2016).

    Google Scholar 

  21. M. Petrovic, D. Bonvin, H. Hofmann and M. Mionic Ebersold, Int. J. Mol. Sci., 19, 1 (2018).

    Google Scholar 

  22. X. C. Li, M. R. Jacob, S. I. Khan, M. K. Ashfaq, K. S. Babu, A. K. Agarwal, H. N. Elsohly, S. P. Manly and A. M. Clark, Antimicrob. Agents. Chemother., 52, 2442 (2008).

    CAS  PubMed  PubMed Central  Google Scholar 

  23. M. Sakko, C. Moore, L. Novak-Frazer, V. Rautemaa, T. Sorsa, P. Hietala, A. Jarvinen, P. Bowyer, L. Tjaderhane and R. Rautemaa, Mycoses, 57, 214 (2014).

    CAS  PubMed  Google Scholar 

  24. S. Puvvada, P. Latha, K. B. Jayalakshmi and S. K. Arul, Int. J. Appl. Dent. Sci., 3, 19 (2017).

    Google Scholar 

  25. R. Nassar and M. Nassar, Int. Arab. J. Antimicrob. Agents, 6, 1 (2016).

    CAS  Google Scholar 

  26. A. K. Yadav, P. Sirohi, S. Saraswat, M. Rani, M. P. Singh, S. Srivastava and N. K. Singh, Curr. Microbiol., 75, 849 (2018).

    CAS  PubMed  Google Scholar 

  27. J. J. Hue, L. Li, Y. E. Lee, K. N. Lee, S. Y. Nam, Y. W. Yun, J. H. Jeong, S. H. Lee, H. S. Yoo and B. J. Lee, J. Food Hyg. Saf., 22, 37 (2007).

    Google Scholar 

  28. N. H. Kim and M. S. Rhee, Appl. Environ. Microbiol., 82, 1040 (2016).

    PubMed  PubMed Central  Google Scholar 

  29. M. Cho, H. Chung and J. Yoon, Environ. Sci. Technol., 37, 2134 (2003).

    CAS  PubMed  Google Scholar 

  30. M. Cho, H. Kim, S. H. Cho and J. Yoon, Ozone Sci. Eng., 25, 251 (2003).

    CAS  Google Scholar 

  31. M. Cho, J. H. Kim and J. Yoon, Water Res., 40, 2911 (2006).

    CAS  PubMed  Google Scholar 

  32. M. J. Karnovsky, J. Cell Biol., 27, A137 (1965).

    Google Scholar 

  33. L. K. Dhandole, Y. S. Seo, S. G. Kim, A. Kim, M. Cho and J. S. Jang, Photochem. Photobiol. Sci., 18, 1092 (2019).

    CAS  PubMed  Google Scholar 

  34. M. D. Sobsey, T. Fuji and P. A. Shields, Water Sci. Technol., 20, 385 (1988).

    CAS  Google Scholar 

  35. A. M. Driedger, J. L. Rennecker and B. J. Mariñas, Water Res., 34, 3591 (2000).

    CAS  Google Scholar 

  36. M. Cho, V. Gandhi, T. M. Hwang, S. Lee and J. H. Kim, Water Res., 45, 1063 (2011).

    CAS  PubMed  Google Scholar 

  37. C. L. Hawkins, D. I. Pattison and M. J. Davies, Amino Acids, 25, 259 (2003).

    CAS  PubMed  Google Scholar 

  38. P. Breeuwer and T. Abee, Int. J. Food Microbiol., 55, 193 (2000).

    CAS  PubMed  Google Scholar 

  39. M. Berney, H. U. Weilenmann and T. Egli, Microbiology, 152, 1719 (2006).

    CAS  PubMed  Google Scholar 

  40. Q. Zhou, Y. Zhao, H. Dang, Y. Tang and B. Zhang, J. Food Prot., 82, 826 (2019).

    CAS  PubMed  Google Scholar 

  41. L. Leive, Biochem. Biophys. Res. Commun., 21, 290 (1965).

    CAS  PubMed  Google Scholar 

  42. M. Vaara, Microbiol. Rev., 56, 395 (1992).

    CAS  PubMed  PubMed Central  Google Scholar 

  43. I. M. Helander and T. Mattila-Sandholm, J. Appl. Microbiol., 88, 213 (2000).

    CAS  PubMed  Google Scholar 

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Acknowledgements

This work was supported by the Korea Environment Industry and Technology Institute (KEITI) through Public Technology Program based on Environmental Policy, funded by Korea Ministry of Environment (MOE) (2018000200001) and by the Korean National Research Foundation (2016M3A7B4909370 and 2018R1 A6A3A01010578).

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  1. These authors equally contributed.

Authors and Affiliations

  1. SELS Center, Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, 54596, Korea

    Young-seok Seo, Nuri Choi, Kangmin Kim & Min Cho

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  1. Young-seok Seo
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  2. Nuri Choi
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  3. Kangmin Kim
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  4. Min Cho
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Correspondence to Min Cho.

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Seo, Ys., Choi, N., Kim, K. et al. Free chlorine and phytic acid synergistically inactivated conidia of Aspergillus spp.. Korean J. Chem. Eng. 36, 1799–1805 (2019). https://doi.org/10.1007/s11814-019-0366-z

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  • DOI: https://doi.org/10.1007/s11814-019-0366-z

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