[EZ-Cytox] Injectable biodegradable gelatin-methacrylate/β‐tricalcium phosphate composite for the repair of bone defects
- - 짧은주소 : http://dogenbio.com/bbs/?t=6z
- - 년도 : 2019
- - 제품명 : EZ-Cytox
- - 학술지명 : Chemical Engineering Journal
- - 주소링크 : https://www.sciencedirect.com/science/article/pii/S1385894719302372
Maintaining appropriate mechanical strength and tissue volume is important to provide for sufficient alveolar bone growth. Recently, synthetic bone graft materials (BGM) including hydroxyapatite (HA) and beta-tricalcium phosphate (β-TCP) have been widely studied for alveolar bone regeneration. Here, we applied gelatin-methacrylate (GelMA) hydrogel into the β-TCP to produce a GelMA/β-TCP composite (GelMA-B). This reinforced the mechanical strength and regulated the absorption period of β-TCP. To characterize the physical properties of the formed composite, GelMA-B was tested using a mechanical tester and a rheometer. It was then analyzed for biocompatibility and osteogenic differentiation using human adipose-derived stem cells. Further, the bone regeneration effects of GelMA-B were confirmed by an animal study. The cell viability analysis and qPCR results revealed that GelMA-B showed high cell viability and relatively high bone differentiation ability. In a rheological analysis, GelMA-B showed higher mechanical strength than β-TCP and GelMA alone. These features were confirmed in the animal study as GelMA-B maintained its volume and shape during regrowth, which can be favorable for alveolar bone regeneration. In addition to the regenerative effect, GelMA-B composite has an advantage for clinical use as the material enables structural molding for customized application.