Proteome Analysis of Human Natural Killer Cell Derived Extracellular Vesicles for Identification of Anticancer Effectors > REFERENCE LIBRARY

본문 바로가기
커뮤니티

[EZ-Cytox] Proteome Analysis of Human Natural Killer Cell Derived Extracellular Vesicles for Identification of …

김상진
2022-01-27 09:30 2,552 1

본문

년도
2020
제품명
EZ-Cytox
학술지명
Molecules

Cancer immunotherapy is a clinically validated therapeutic modality for cancer and has been rapidly advancing in recent years. Adoptive transfer of immune cells such as T cells and natural killer (NK) cells has emerged as a viable method of controlling the immune system against cancer. Recent evidence indicates that even immune-cell-released vesicles such as NK-cell-derived exosomes also exert anticancer effect. Nevertheless, the underlying mechanisms remain elusive. In the present study, the anticancer potential of isolated extracellular vesicles (EVs) from expanded and activated NK-cell-enriched lymphocytes (NKLs) prepared by house-developed protocol was evaluated both in vitro and in vivo. Moreover, isolated EVs were characterized by using two-dimensional electrophoresis (2-DE)-based proteome and network analysis, and functional study using identified factors was performed. Our data indicated that the EVs from expanded and active NKLs had anticancer properties, and a number of molecules, such as Fas ligand, TRAIL, NKG2D, β-actin, and fibrinogen, were identified as effector candidates based on the proteome analysis and functional study. The results of the present study suggest the possibility of NK-cell-derived EVs as a viable immunotherapeutic strategy for cancer.

댓글목록1

김상진님의 댓글

김상진
2022-01-27 09:30
JournalImpactFactor(2019) : 3.267
카카오톡
이메일
견적/제품문의
샘플신청
게시판 전체검색